研究领域/AREAS OF RESEARCH
植物分子生物学研究:
-功能基因组学
-基因工程
马铃薯研究:
-马铃薯块茎分化与发育调控的分子基础
-马铃薯花色苷代谢、遗传与基因工程
-马铃薯抗病虫基因工程
-马铃薯生物技术
-马铃薯遗传资源收集、开发与利用
主要学术贡献/MAJOR ACADEMIC CONTRIBUTIONS
生物技术研发与数据库构建方面:
1. 首次研发成功花椰菜花药培养单倍体再生技术及高效体系;
2. 发明花蕾培养单倍体诱导技术,该技术是继花药培养和小孢子培养技术后第三个雄性生殖单倍体诱导技术;
3. 研制一种野生马铃薯微型薯诱导技术,为野生马铃薯的繁殖和保存提供了新的途径;
5. 利用叶绿体基因工程,在烟草中成功合成长链聚羟基脂肪酸酯;
6. 采用RNAi技术,构建OsGA20ox2 gene 沉默矮杆水稻;
7. 分别应用DDRT-PCR、AFLP-cDNA、RNA-Seq技术建立了3个转录组数据库:1)基于StCO转基因材料的马铃薯块茎分化与发育相关基因表达数据库,2)与黄萎病抗性相关的茄子功能基因转录组数据库,3)与黄萎病抗性相关的茄子小RNA数据库;
马铃薯研究方面:
8. 研究了二倍体马铃薯Solanum Chacoense自交不亲和性,揭示其不亲和机制;
9. 深入研究了马铃薯块茎形成调控的分子基础,鉴定并克隆了多个与马铃薯块茎分化与发育调控相关基因如PHYA、PHYB、COL、FT、TFL等基因,并揭示了它们在块茎形成调控中的功能;
10. 系统地研究了马铃薯花色苷生物合成关键酶基因,克隆了全套结构基因及部分调节基因,并将部分基因成功地应用于马铃薯花色苷含量改良;
11. 克隆了马铃薯类胡萝卜素关键基因StLCYb,并成功地将之应用于马铃薯块茎类胡萝素合成改良;
12. 克隆了数十个马铃薯耐热、耐寒、耐盐、抗虫等基因,并阐明了这些基因的功能;
13. 通过体外抑癌实验,揭示了彩色马铃薯花色苷和绿原酸在抗肝癌和结肠癌中的作用;
14. 开展抗菌肽MSI-99m的应用研究,揭示其在抗马铃薯晚疫病、青枯病、油菜菌核病中的功能;
15. 采用转录组测序,研究了马铃薯栽培品种在热胁迫下的分子响应,揭示了马铃薯耐热的分子基础;
16. 采用生物信息学和分子生物学方法,鉴定了马铃薯HSF家族基因,该家族包27 成员;
17. 采用生物信息学和分子生物学方法,鉴定了马铃薯bZIP家族基因,该家族包63成员;
18. 采用生物信息学和分子生物学方法,鉴定了马铃薯WDR家族基因,该家族包168成员;
茄子研究方面:
19. 系统地研究了受黄萎病菌诱导的野生茄子差异基因表达谱,揭示了野生茄子Solanum torvum抗黄萎病的分子基础,在此基础上克隆和研究了10多个基因的抗病功能;
20. 系统地研究了受黄萎病菌诱导表达的茄子miRNA,揭示了miRNA参与茄子黄萎病防卫过程,研究并揭示了6个miRNA的功能;
21. 采用降解组测序,系统地鉴定了受黄萎病菌诱导表达的茄子miRNA靶基因,发现这些靶基因与小RNA之间在功能上存在的复杂网络关系;
大豆研究方面:
22. 通过转录组测序分析,揭示了大豆对斜纹夜蛾响应在基因和转录水平上的多样性;
23. 采用基于多拷贝分析,研究了大豆异黄酮途径的进化,发现代谢途径的结构和功能决定了基因复制和组成酶的进化约束。
近期部分论文/RECENT SELECTED PUBLICATIONS
- Tang RM, Dong HT, He LH, Li P, Shi YR, Yang Q, Jia XY and Li XQ (2022) Genome?wide identification, evolutionary and functional analyses of KFB family members in potato. BMC Plant Biology (2022) 22:226. https://doi.org/10.1186/s12870-022-03611-y
- Chen M, Xie C, Yang Q, Zhu WJ and Wang M (2022) Protein extract of tobacco expressing Solanum torvum PP5-Encoding Gene Inhibits Verticillium dahliae Proliferation. Horticulturae, 8, 252. https://doi.org/10.3390/horticulturae8030252
- Cheng PF, Wang J, Zhao ZS, Kong LS, Lou W, Zhang T, Jing DD, Yu JL, Shu ZL, LHuang LQ, Zhu WJ, Yang Q and Shen WB (2021) Molecular hydrogen increases quantitative and qualitative traits of rice grain in field trials. Plants, 10, 2331. https://doi.org/10.3390/plants10112331
- Zhu WJ, Liu XR, Chen M, Tao NJ, Tendu A, Yang Q (2021) A new miRNA miRm0002 in eggplant participates in the regulation of defense responses to Verticillium wilt. Plants, 2021, 10(11), 2274. https://doi.org/10.3390/plants10112274
- Zhang GD, Tang RM, Niu SY, Si HJ, Yang Q, Rajora OP, Li XQ (2021) Heat-stress-induced sprouting and differential gene expression in growing potato tubers: Comparative transcriptomics with that induced by postharvest sprouting. Horticulture Research, 8:226, https://doi.org/10.1038/s41438-021-00680-2
- Li T, Tao NJ, Lin YM, Chen M, Jiao DL, Xue CM, Zhu WJ, Yang Q (2021) Identification of miRNA targets in eggplant in response to Verticillium dahliae by degradome sequencing. Eur J Plant Pathol, https://doi.org/10.1007/s10658-021-02217-8
- Zhu WJ, Li PP, Xue CM, Chen M, Wang Z, Yang Q (2021) Potato plants overexpressing SpHsfA4c exhibit enhanced tolerance to high-temperature stress. Russian Journal of Plant Physiology, https://doi.org/10.1134/S1021443721060248
- Zhu WJ, Jiao DL, Zhang J, Xue CM, Chen M, Yang Q (2020) Genomewide identification and analysis of BES1/BZR1 transcription factor family in potato (Solanum tuberosum L). Plant Growth Regulation, https://doi.org/10.1007/s10725-020-00645-w
- Tang RM, Gupta SK, Niu SY, Li XQ, Yang Q, Chen GS, Zhu WJ, Haroon M (2020) Transcriptome analysis of heat stress response genes in potato leaves. Molecular Biology Reports, https://doi.org/10.1007/s11033-020-05485-5
- Zhang GD, Tang RM, Niu SY, Si HJ, Yang Q, Bizimungu B, Regan S, Li XQ (2019) Effects of earliness on heat stress tolerance in fifty potato cultivars. American Journal of Potato Research, https://doi.org/10.1007/s12230-019-09740-9
- Tao NJ, Zhu WJ, Gan MJ, Chen M, Li T, endu A, Jiao DL, Wang M, Xue CM, Lin YM, Yang Q (2019) Genomewide identification and functional analysis of the WDR protein family in potato. 3 Biotech, 9:432, https://doi.org/10.1007/s13205-019-1965-4
- Wang T, Chen YM, Zhuang WB, Zhang FJ, Shu XC, Wang Z, Yang Q (2019) Transcriptome Sequencing Reveals Regulatory Mechanisms of Taxol Synthesis in Taxus wallichiana var. Mairei. International Journal of Genomics, https://doi.org/10.1155/2019/1596895
- Zhu WJ, Bai X, Li GT, Chen M, Wang Z, Yang Q (2019) SpCYS, a cystatin gene from wild potato (Solanum pinnatisectum), is involved in the resistance against Spodoptera litura. Theor. Exp. Plant Physiol., 31:317–328, https://doi.org/10.1007/s40626-019-00148-8
- Tang RM, Niu SY, Zhang GD, Chen GS, Haroon M, Yang Q, Rajora OP, Li XQ (2018) Physiological and growth responses of potato cultivars to heat stress. Botany, 96: 897-912, https://doi.org/10.1139/cjb-2018-0125
- Zhu WJ, Shi K, Tang RM, Mu XY, Cai JH, Chen M, You X, Yang Q (2018) Isolation and functional characterization of the SpCBF1 gene from Solanum pinnatisectum. Physiol Mol Biol Plants, 24(4):605-616, https://doi.org/10.1007/s12298-018-0536-1
- Mu XY, Liu XR, Cai JH, Zhu WJ, Wang Z, Yang Q, You X (2018) MiR395 overexpression increases eggplant sensibility to Verticillium dahliae infection. Russ J of Plant Physiololy, 65(2): 203-210
- Liu L, Zhang RJ, Zhu WJ, Liu XR, Shi K, Chen M, Yang Q (2017) Inhibitory effect of StCYP707A1 gene on tuberization in transgenic potato. Plant Biotechnol Rep, 11: 219-228, DOI: 10.1007/s11816-017-0442-y
- Tang RM, Zhu WJ, Song XY, Lin XZ, Cai JH, Wang M, Yang Q (2016) Genome-wide identification and function analyses of heat shock transcription factors in potato. Front. Plant Sci., 7:490. https://doi:10.3389/fpls.2016.00490
- Song XY, Zhu WJ, Tang RM, Cai JH, Chen M, Yang Q (2016) Over-expression of StLCYb increases b-carotene accumulation in potato tubers. Plant Biotechnol Rep, 10:95-104, DOI: 10.1007/s11816-016-0390-y
- Song XY, Zhang CZ, LI Y, Feng SS, Yang Q, Huang SW (2016) SSR analysis of genetic diversity among 192 diploid potato cultivars. Horticultural Plant Journal, 2 (3): 163-171.
- Chen M, Zhu WJ, You X, Liu YD, Kaleri GM, Yang Q (2015) Isolation and characterization of a chalcone isomerase gene promoter from potato cultivars. Genetics and Molecular Research, 14 (4): 18872-18885, http://dx.doi.org/10.4238/2015.December.28.37
- Wang SH, Yang XY, Xu MN, Lin XZ, Lin T, Qi JJ, Shao GJ, Tian NN, Yang Q, Zhang ZH, Huang SW (2015) A Rare SNP Identified a TCP Transcription Factor Essential for Tendril Development in Cucumber. Molecular Plant, 10/2015; DOI:10.1016/j.molp.2015.10.005
- Wang YL, Wang H, Ma YJ, Du HP, Yang Q, Yu DY (2015) Identification of transcriptional regulatory nodes in soybean defense networks using transient co-transactivation assays. Frontiers in Plant Science, 6: 1-16, http://dx.doi.org/10.3389/fpls.2015.00915
- Wang YL, Wang H, Ma YJ, Yang WM, Yang Q, Yu DY (2015) Identification of soybean herbivoryregulated genes and a transgenic investigation of their potential in insect resistance. Plant Cell Tissue and Organ Culture, 08/2015; 123(2):321-340, DOI:10.1007/s11240-015-0837-9
- Yang L, Shi C, Mu XY, Liu C, Shi K, Zhu WJ, Yang Q (2015) Cloning and expression of a wild eggplant cytochrome P450 gene, StoCYP77A2, involved in plant resistance to Verticillium dahliae. Plant Biotechnol Rep, 9:167-177, DOI 10.1007/s11816-015-0355-6
- Yang L, Mu XY, Liu C, Cai JH, Shi K, Zhu WJ and Yang Q (2015) Overexpression of potato miR482e enhanced plant sensitivity to Verticillium dahliae infection. Journal of Integrative Plant Biology, 57(12):1078-1088
- Jue DW, Yang L, Shi C, Chen M, Yang Q (2014) Cloning and characterization of a Solanum torvum NPR1 gene involved in regulating plant resistance to Verticillium dahliae. Acta Physiol Plant, 36:2999-3011, DOI 10.1007/s11738-014-1671-0
- Chu SS, Wang J, Cheng H, Yang Q, Yu DY (2014) Evolutionary study of the isoflavonoid pathway based on multiple copies analysis in soybean. BMC Genetics, 15:76, http://www.biomedcentral.com/1471-2156/15/76
- WANG YL, WANG H, FAN R, YANG Q, YU DY (2014) Transcriptome analysis of soybean lines reveals transcript diversity and genes involved in the response to common cutworm (Spodoptera litura Fabricius) feeding. Plant, Cell & Environment, 37: 2086-2101, doi: 10.1111/pce.12296
- Li W, Wang B, Wang M, Chen M, Yin JM, Kaleri GM, Zhang RJ, Zuo TN, You X and Yang Q (2014) Cloning and characterization of a potato StAN11 gene involved in anthocyanin biosynthesis regulation. Journal of Integrative Plant Biology, 56(4): 364-372, DOI: 10.1111/jipb.12136
- Zhang YF, Yang Q, Ji Q, Wang DZ, Xu C (2014) Expression, prification, and synergistic effects in starch binding of starch-binding domains (SBD-SBD4) derived from Aspergillus niger 2316 glucoamylase. Journal of pure and applied microbiology, 8(3):2021-2030
- Sang XL, Jue DW, Yang L, Bai X, Chen M, Yang Q (2013) Genetic transformation of Brassica napus with MSI-99m gene increases resistance in transgenic plants to Sclerotinia sclerotiorum. Molecular Plant Breeding, 4(30): 247-253, doi: 10.5376/mpb.2013.04.0030
- Yang L, Xie C, Li W, Zhang RJ, Jue DW, Yang Q (2013) Expression of a wild eggplant ribosomal protein L13a in potato enhances resistance to Verticillium dahliae. Plant Cell Tiss Organ Cult, 115(3) : 329-340, DOI 10.1007/s11240-013-0365-4
- Yang L, Jue DW, Li W, Zhang RJ, Chen M, Yang Q (2013) Identification of miRNA from eggplant (Solanum melongena L.) by small RNA deep sequencing and their response to Verticillium dahliae infection. PLoS ONE, 8(8): e72840. doi:10.1371/journal.pone.0072840
- Liu SP, Hong YB, Wu Z, Ma YS, Jue DW, Xie C, Zhu YP, Chen M and Yang Q (2013) Protein extract of tobacco expressing StoVe1 gene inhibits Verticillium dahliae proliferation. Czech J. Genet. Plant Breed., 49: 58–64
- Hong YB, Liu SP, Zhu YP, Xie C, Jue DW, Chen M, Kaleri HA and Yang Q (2013) Expression of the MSI-99m gene in transgenic potato plants confers resistance to Phytophthora infestans and Ralstonia solanacearum. Plant Mol Biol Rep, 31:418-424
- Liu SP, Zhu YP, Xie C, Jue DW, Hong YB, Chen M, Hubdar AK, Yang Q(2012)Transgenic potato plants expressing StoVe1 exhibit enhanced resistance to Verticillium dahliae. Plant Mol Biol Rep, 30:1032-1039
- Wei Q, Feng ZH, Wang QY, Wang B, Zhang YF, Yang Q (2012) Increased accumulation of anthocyanins in transgenic potato tubers by over-expression of 3GT gene. Plant Biotechnol Rep,6: 69-75
- Wang QY, Chen Q, He ML, Mir PY, Su JY and Yang Q (2011): Inhibitory effect of antioxidant extracts from various potatoes on the proliferation of human colon and liver cancer cells, Nutr Cancer, 63(7):1044-1052, http://www.tandfonline.com/loi/hnuc20
- Xie YJ, Cui WT, Yuan XX, Shen WB, Yang Q (2011) Haem oxygenase-1 is associated with wheat salinity acclimation by modulating reactive oxygen species homeostasis. Journal of Integrative Plant Biology, 53( 8): 653-670, www.wileyonlinelibrary.com/journal/jipb
- Xie YJ, Xu S, Han B, Wu MZ, Yuan XX, Han Y, Gu Q, Xu DK, Yang Q and Shen WB (2011) Evidence of Arabidopsis salt acclimation induced by up-regulation of HY1 and the regulatory role of RbohD-derived reactive oxygen. species synthesis. Plant Journal, 66(2):280-292, https://doi: 10.1111/j.1365-313X.2011.04488.x
授权专利/PATENTS
1) 杨清,史策,陈敏,决登伟. 野生茄子细胞色素氧化酶基因StCYO77A2及其表达载体和应用. 专利号:2013.1.0147109.3, 国家发明专利,2014。
2)杨清,决登伟,陈敏,杨柳. 野生茄子StoNPR1基因及其应用. 专利号:2013,1,0290069.8, 国家发明专利,2015。
3)杨清,决登伟,陈敏. 野生茄子StoWRKY6基因及其表达载体和应用. 专利号:2013.1.0549678,0, 国家发明专利,2015。
奖励与荣誉/ AWARDS AND HONORS
2012年:“细胞分子生物学”课程获得江苏省教育厅优秀研究生课程奖励;
2012年:获得江苏省侨联颁发的江苏省归侨先进个人称号;
2018年:在中国马铃薯大会上获得优秀论文一等奖。
社会兼职/PROFESSIONAL AFFILIATIONS
2002年,淮阴工学院兼职教授;
2006年,江苏农林职业技术学院园艺专业指导委员会委员;
2021年,国际学术期刊“GENES”编委。
服务地方/SOCIAL WORK
1)参与江苏省马铃薯科技示范园建设,作为首席专家,参与了示范园项目的申报,园区设计和建设过程;
2)开展马铃薯品种资源的引进与试验评价研究,针对江苏地区的气候特点,从国内外引进一批早熟优良的马铃薯品种,并在江苏条件下进行品种特性和农艺性状的评价试验,筛选适合于本地种植的马铃薯品种;
3)开展马铃薯产业化宣传与推广工作,多次接收平面媒体和电视媒体的采访,介绍马铃薯的营养价值和经济价值,宣传马铃薯对江苏地区农业经济的重要性和马铃薯主粮化的发展前景,经网络大量传播后产生了积极的社会影响。
科研合作/SCIENTIFIC RESEARCH COOPERATION
- 加拿大农业与农业食品部(AAFC)马铃薯研究中心,Dr Li Xiu-Qing, 资深研究员;
- 中国农业科学院副经理,深圳应用基因组研究所所长,黄三文研究员。
联系方式/CONTACT INFORMATION
地址:江苏省南京市玄武区卫岗1号, 邮编:210095; Tel: 025-84395221 (O), E-mail: qyang19@njau.edu.cn
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植物基因工程与生物技术实验室 https://weibo.com/7591411398/LDt0Am7fd